Wintrobe Tubes Glass

  • Wintrobe Tubes Glass 
  • Size:4.5 inch
  • Country of Origin: India


What are the uses of Wintrier tube

The uses are
1.Determination of Packed cell volume (PCV)
2.Determination of Erythrocyte sedimentation rate (ESR)
3.Preparation of Buffy coat smear.

How do you estimate PCV by wintrobe method
a.Fill the pasteur pipette with properly mixed anticoagulated blood .
b.Introduce the pipette at the bottom of the wintrobe tube and slowly fill it upto 10 mark.
c.Make sure that the tip of pipette should remain below the rising meniscus of blood to avoid foaming.
d. Centrifuge the tube at 3000 rpm for 30 min.
Upper most layer is PLASMA
Middle narrow layer is BUFFY COAT
Lower most layer is the PACKED RBCs

What are the normal values of PCV
NORMAL VALUES are as follows
Adult male – 40 – 50%
Adult female – 38 – 45%
New born – 44 – 60%

Why is the PCV test asked by the clinician
Apart for this, the other reasons are to monitor the treatment of anemias, polycythemia and any dehydration

What are the causes of increased PCV
The causes of Increased PCV are
The most common cause of increased PCV is dehydration
Other causes include
Polycythemia: Primary or secondary
Some causes of secondary polycythemia
Lung or heart diseases: there will be increased production of RBC’s to meet the oxygen demands
Smoking and alcohol consumption
Paraneoplastic syndromes where there is increase in erythropoietin levels: Eg Hepatocellular carcinoma, Renal cell carcinoma, Adrenal tumors etc

What are the causes of decreased PCV
Anemias and leukemias
Secondary to bleeding
A small drop may be seen in pregnancy
Renal diseases may result in decrease in production of erythropoietin



1st floor shop #13 kader tropical

10 shaid nazrul islam road, hatkhola, tikatoly Dhaka-1203

E-mail:, Website: WWW.ASTZBD.COM

Cell: +88 01329100999

        +88 01329100998


There are no reviews yet.

Be the first to review “Wintrobe Tubes Glass”